Genemore G-MM-IGT Biotinylation Kit

Genemore G-MM-IGT Biotinylation Kit

①: Easy to use and operate
②: Reusable multiple times
③: Strict quality control
④: Widely used in ELISA, EMSA, biosensor technology, and other applications
产品详情

Biotinylation is one of the most popular labeling methods and is widely used in ELISA, EMSA, biosensor technology, and other interaction detection techniques. The biotinylation reagent in this kit is primarily based on amine-based biotinylation, where biotin is covalently coupled to the amino group (-NH2) of the target substance. For example, in the case of proteins, as shown in the diagram below:

After biotinylation is completed, gel filtration (desalting column) is used to remove unreacted small-molecule biotinylation reagents and to collect the labeled macromolecules.

This kit includes a 20 µL tube of 10 mM biotinylation reagent and a desalting column (with a molecular weight cutoff of 5000 Da).

Applications**: Biotinylation and purification of substances containing amino groups.


01. Easy to Use and Operate  

The biotinylation process is completed within 1 hour and does not require special equipment.

02. Reusable  

Can be used more than 20 times.

03. Strict Quality Control  

Genemore undergoes strict quality control and guarantees excellent biotinylation efficiency, as verified through biosensor technology.


1. Why is the biotinylation efficiency low?

a) Excess biotinylation reagent was not completely removed, and dialysis or re-purification through a desalting column may be required to remove free biotin.

b) The concentration of the target substance before biotinylation is too low.

c) Other substances containing amino groups are present in the sample. Remove other amino-containing substances and then proceed with biotinylation.

2. How should I perform biotinylation if my molecule is smaller than 5000 Da?

You can mix the target substance and biotinylation reagent at a molar ratio of 5-10 times, which may ensure that the biotinylation reagent reacts completely. In this case, you do not need to use a desalting column to remove unreacted biotinylation reagent.

3. How many times can the biotinylation reagent be used? How many times can the desalting column be reused?

A 20 µL biotinylation reagent can typically be used for 10-20 times. In principle, the desalting column can be reused infinitely. However, if you notice a slower flow rate, it may indicate that the desalting column needs to be replaced.

4. What are the requirements for the target substance to be biotinylated?

The target substance must meet the conditions listed under "Target substances to be biotinylated" in the "Experimental reagents and consumables" section, and it must contain amino groups.